Spectrophotometric determination of catechol amine drugs in pharmaceutical preparations via oxidative coupling reaction with 3-amino pyridine and sodium periodate

Abstract EN

A rapid and sensitive spectrophotometric method is described for the determination of some Catechol amine drugs (methyldopa (I), adrenaline (II), and dopamine (III)) in both pure and dosage forms.

The proposed method uses 3-amino pyridine as a chromogenic reagent.

The method is based on the oxidative coupling reaction of Catechol amine drugs with 3-amino pyridine and sodium periodate to form an orange-water-soluble dye product, that has a maximum absorption at 476, 488 and 490 nm for (I), (II), and (III) respectively.

The optimum reaction conditions and other analytical parameter are evaluated.

The proposed method was applied successfully to the determination of these drugs in pharmaceutical formulation.

The results have demonstrated that the method is equally accurate and reproducible as the official methods.