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Isolation of stem cells from the mid gut epithelium of culex pipiens mosquitoes (diptera : culicidae)
Joint Authors
Wasim, N. M.
Sulayman, B. A.
Husayn, M. I.
Mutawalli, H. G.
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 44, Issue 1 (30 Apr. 2014), pp.13-20, 8 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2014-04-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Topics
Abstract EN
-Stem cells are undifferentiated cells that remarkable ability to self-renew and produce differentiated cells that support normal development and tissue homeostasis.
The authors isolated spherical stem cells from the midgut epithelium of the late third larval instars of Culex pipiens mosquitoes.
In vitro, cultivated epithelial stem cells were able to proliferate and differentiate in fresh culture for 48 hr in absence of 20- hydroxyecdysone (20-E).
The stem cells were the most common cells in the initial culture.
One hundred of epithelial stem cells had been cultured.
After 24 hr became 132 cells, 78% was undifferentiated stem cells and 8.3% &13.7% was enterocytes and goblet cells respectively.
This ratio slightly decreased after de 48 hr to be 74% undifferentiated stem cells, 4.5 % enterocytes and 5.6 % goblet cells.
After 72 hr the undifferentiated stem cells were not able to proliferate .The most differentiated goblet and enterocytes survived few days then died.
American Psychological Association (APA)
Wasim, N. M.& Sulayman, B. A.& Husayn, M. I.& Mutawalli, H. G.. 2014. Isolation of stem cells from the mid gut epithelium of culex pipiens mosquitoes (diptera : culicidae). Journal of the Egyptian Society of Parasitology،Vol. 44, no. 1, pp.13-20.
https://search.emarefa.net/detail/BIM-363814
Modern Language Association (MLA)
Wasim, N. M.…[et al.]. Isolation of stem cells from the mid gut epithelium of culex pipiens mosquitoes (diptera : culicidae). Journal of the Egyptian Society of Parasitology Vol. 44, no. 1 (Apr. 2014), pp.13-20.
https://search.emarefa.net/detail/BIM-363814
American Medical Association (AMA)
Wasim, N. M.& Sulayman, B. A.& Husayn, M. I.& Mutawalli, H. G.. Isolation of stem cells from the mid gut epithelium of culex pipiens mosquitoes (diptera : culicidae). Journal of the Egyptian Society of Parasitology. 2014. Vol. 44, no. 1, pp.13-20.
https://search.emarefa.net/detail/BIM-363814
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 18-20
Record ID
BIM-363814