Use of bee honey as a carbon source in banana (Musa spp.)‎ tissue culture propagation medium

Dissertant

Ali, Sayf al-Nasr al-Nur Abd Allah

Thesis advisor

Mahdi, al-Fatih Muhammad
Abd al-Rahim, Jamal al-Din al-Tayyib

University

Omdurman Islamic University

Faculty

Faculty of Agriculture

Department

Department of Horticulture

University Country

Sudan

Degree

Master

Degree Date

2012

English Abstract

This study was carried out to investigate the effect of bee honey (as carbon source) on in vitro growth, rate of multiplication and roots formation, leaf mineral contents and histological characteristics of the leaves during acclimatization stage of banana cv.

Grand Nain.

Different concentration of bee honey (14.51, 29.02, 43.53, 58.04, 72.54 and 87.05 g / l) were tested.

Two controls were used in multiplication stage; control 1 (banana basic medium (BBM) with 30 g / l sucrose) and control 2 (BBM without carbon source) while, control in rooting stage was BBM with 20g/l sucrose.

The results showed that sterilization by autoclaving affected bee honey sugars content.

However, the results indicated that the plants cultured on BBM containing 30 g / l sucrose (control 1) were taller than all plants of other treatments in the first three multiplication cycles whereas, BBM + 43.53 g / l bee honey gave the highest plant height in fourth multiplication cycle.

The highest number of shoots was observed in plants derived from BBM + 58.04 g / l bee honey in first and third multiplication cycles; BBM + 14.51g / l bee honey and BBM with 30 g/l sucrose (Control 1) in second and fourth multiplication cycles respectively.

The maximum number of leaves was recorded in plants cultured on BBM with 29.02g/l bee honey in second and fourth multiplication cycles and BBM with 30 g/l sucrose (Control 1) in first and third multiplication cycles.

At the end of multiplication stage (four months), the maximum mean number of shoots was obtained in plantlets cultured on BBM + 58.04 g / l bee honey followed by plantlets cultured on BBM with 30 g/l sucrose (Control 1) and BBM + 43.53 g / l bee honey.

The greatest values of growth parameters measured were recorded in BBM with 30 g / l sucrose (Control 1).

The highest survival percentage (100 %) was recorded in plants transferred from BBM supplemented with 72.54 g / l bee honey, followed by (90 %) in BBM+29.02 g / l bee honey.

The plants transferred from BBM containing lower bee honey concentrations recorded the highest values in leaf P and Mg content.

Plants derived from BBM containing bee honey at concentration 43.53 to 72.54 g / l gave higher roots length, plants height and leaf N, K, Mn, Fe and Zn contents.

The highest Ca and Cu concentrations in leaf were obtained in BBM with 29.02 g / l and control (BBM + sucrose) respectively.

The highest number of leaves and roots was recorded on plants transferred from BBM supplemented with bee honey at concentration 43.53 g / l and also BBM with 87.05 g/l recorded the same leaves number. The leaves cross sections showed that the thickest upper epidermis and hypodermis layers were observed in leaves of plant cultured on BBM supplemented with 29.02 g / l bee honey.

Moreover, the thickest lower epidermis layer, spongy tissue and leaf thickness were recorded in leaves of control plants (BBM with sucrose).

On the other hand, the thickest palisade tissues were observed in leaves of plants cultured on BBM with bee honey at higher concentration.

Lower thickness values of leaf and hypodermis layers were observed in leaf of plants produced on BBM with 58.04 to 87.05 g / l bee honey.

In addition, leaf of plants produced on BBM+58.04 g / l bee honey had thinner epidermis layers.

The thickest mesophyll recorded in leaf plants cultured on BBM supplemented with 29.02 and 43.53 g / l bee honey respectively.

While, longitudinal sections of shoots apices indicated that the highest values of tunica thickness, apical dome depth and diameter were observed in plants cultured on BBM containing 43.53 g / l bee honey followed by plants cultured on BBM with 72.54 g / l bee honey.

Also the deepest corpus was recorded in plants derived from BBM supplemented with 72.54 g / l and 43.53 g / l bee honey respectively. The lowest values of plants survival percentage (70.59 %, 68.75 %), roots number and length and shoot apices layers size were observed in plants transferred from BBM supplemented with bee honey at lower (14.51g / l) and higher (87.05 g / l) concentrations.

Moreover, the plants transferred from BBM containing lower bee honey concentration recorded the minimum values in plant height, number of leaves, leaf N, Ca, and micronutrients content.

While, the lowest values of multiplication and growth parameters measured in vitro were obtained in plants cultured on BBM without carbon source (control 2), BBM with bee honey at lower (14.51 g / l) and higher (87.05 g / l) concentration respectively.

Main Subjects

Botany

Topics

• Bananas

No. of Pages

138

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-364124