Main properties of urease partially purified from seeds of syrian mesquite (prosopis farcta)
Author
Source
Journal of Babylon University : Journal of Applied and Pure Sciences
Issue
Vol. 22, Issue 3 (30 Jun. 2014), pp.1071-1079, 9 p.
Publisher
Publication Date
2014-06-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract AR
تم اختبار فعالية اليوريز من مصادر جديدة لنباتات العائلة البقولية حيث وجدت أعلى فعالية نوعية في نبات الخرنوب (52-36 وحدة / ملغم).
نقي المستخلص الفعال بعدة خطوات شملت التمليح الخارجي، المبادل الأيوني السالب و الموجب و الترشيح الهلامي حيث كانت الفعالية النوعية 44-52، 27-193، 55-23 و 33-433 وحدة / ملغم على التوالي.
حددت الخصائص الرئيسية لانزيم اليوريز مثل حساب الوزن الجزئي (95,500 كيلو دالتون)، الأس الهايدروجيني الأمثل للفعالية (٨) و إثبات (7-8)، درجة الحرارة المثلى للفعالية (٤٥ مₒ و الثبات (25-30 مₒ).
Abstract EN
Novel sources of urease from leguminosae were selected for activity test, one the them (Prosopis farcta) showed high specific activity (36.52 U mg-1).
The active uerase extract then subjected to series of purification steps involved salting out, DEAE, CM ion exchange and size exclusion chromatography.
Specific activity were 52.44, 193.27, 230.55, 433.33 U mg-1 respectively.
Main features of urease were detected included estimation of molecular weight (95.500 kDa), optimum pH for activity (8) and stability (7-8), optimum temperature for activity (45oC) and stability (25-30 oC).
American Psychological Association (APA)
Hamzah, Nizar Abd al-Amir. 2014. Main properties of urease partially purified from seeds of syrian mesquite (prosopis farcta). Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 22, no. 3, pp.1071-1079.
https://search.emarefa.net/detail/BIM-364218
Modern Language Association (MLA)
Hamzah, Nizar Abd al-Amir. Main properties of urease partially purified from seeds of syrian mesquite (prosopis farcta). Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 22, no. 3 (2014), pp.1071-1079.
https://search.emarefa.net/detail/BIM-364218
American Medical Association (AMA)
Hamzah, Nizar Abd al-Amir. Main properties of urease partially purified from seeds of syrian mesquite (prosopis farcta). Journal of Babylon University : Journal of Applied and Pure Sciences. 2014. Vol. 22, no. 3, pp.1071-1079.
https://search.emarefa.net/detail/BIM-364218
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1078-1079
Record ID
BIM-364218
