The use of microencapsulated hepatocytes transplantation reduces mortality and liver alterations in schistosoma mansoni infected hamsters
Joint Authors
Muharib, Muna N.
Sharif, Suad A.
al-lakkanyn, Najla M.
Hammam, Ulfat A.
Salman, Fatimah H.
al-Najjar, Muhammad M.
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 44, Issue 1 (30 Apr. 2014), pp.229-242, 14 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2014-04-30
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Topics
Abstract EN
-Hepatocyte transplantation is an attractive therapeutic modality for liver disease as an alternative for orthotropic liver transplantation.
The goal of this work was to study the adequacy of intrasplenic hepatocyte transplantation (HCTx) in fresh and microencapsulated forms, in a hamster model of liver fibrosis by Schistosoma mansoni infected hamsters were divided into 6 groups; untreated for 11 weeks (GI) and for 15 weeks (GII), treated with praziquantel (PZQ) 7 weeks PI, and killed 4 weeks (GIII) and 8 weeks (GIV) post-treatment.
Treated with PZQ 7 weeks PI, and then treated orally with immunosuppressive drug "cyclosporine (4 weeks post PZQ treatment), 24 hr.
before interasplenic injection with fresh hepatocytes (V).
Treated with PZQ 7 weeks PI, and then injected interasplenically (4 weeks post-treatment) with microencapsulated hepatocytes (GVI).
GI & GIII were killed 11 weeks PI for assessment the anti-schistosomal efficacy of PZQ.
The other four groups were killed 15 weeks PI for investigation of liver and spleen histology, serum liver enzymes and hepatic oxidative markers before and after HCTx.
Freshly isolated hepatocytes with a mean viability 92.971.2% were used for microencapsulation and transplantation.
Histological study showed the presence of transplanted hepatocytes in spleen of recipient.
PZQ accelerated healing of hepatic granulomatous lesions as evidenced parasitologically by the increase in the percentage of dead eggs and histologically showing more granuloma circumscription with more ova degeneration and less inflammatory cells.
The 25-day survival rates in GII, GIV, GV& GVI were 5/15 (33.3%), 8/15 (53.3%), 10/15 (66.7%) and 9/15 (60%) respectively.
In addition, there were significantly better outcomes in serum biochemical indexes such as ALT, AST, -GT, ALP, and hepatic SOD and MDA in the fresh and microencapsulated groups than in PZQ-treated group, without great differences between the microencapsulated and the fresh transplanted groups.
Liver pathological staining supported these findings.
American Psychological Association (APA)
Sharif, Suad A.& Muharib, Muna N.& al-lakkanyn, Najla M.& Hammam, Ulfat A.& Salman, Fatimah H.& al-Najjar, Muhammad M.. 2014. The use of microencapsulated hepatocytes transplantation reduces mortality and liver alterations in schistosoma mansoni infected hamsters. Journal of the Egyptian Society of Parasitology،Vol. 44, no. 1, pp.229-242.
https://search.emarefa.net/detail/BIM-364388
Modern Language Association (MLA)
Sharif, Suad A.…[et al.]. The use of microencapsulated hepatocytes transplantation reduces mortality and liver alterations in schistosoma mansoni infected hamsters. Journal of the Egyptian Society of Parasitology Vol. 44, no. 1 (Apr. 2014), pp.229-242.
https://search.emarefa.net/detail/BIM-364388
American Medical Association (AMA)
Sharif, Suad A.& Muharib, Muna N.& al-lakkanyn, Najla M.& Hammam, Ulfat A.& Salman, Fatimah H.& al-Najjar, Muhammad M.. The use of microencapsulated hepatocytes transplantation reduces mortality and liver alterations in schistosoma mansoni infected hamsters. Journal of the Egyptian Society of Parasitology. 2014. Vol. 44, no. 1, pp.229-242.
https://search.emarefa.net/detail/BIM-364388
Data Type
Journal Articles
Language
English
Notes
Includes appendices : p. 239-242
Record ID
BIM-364388