Purification and characterization of membrane-bound peroxidase from date palm leaves (Phoenix dactylifera L.)
Joint Authors
Ismail, Muhammad A.
al-Sunaydi, Abd al-Rahman M.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 18, Issue 3 (30 Sep. 2011), pp.293-298, 6 p.
Publisher
Publication Date
2011-09-30
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Abstract EN
Peroxidase from date palm (Phoenix dactylifera L.) leaves was purified to homogeneity and characterized biochemically.
The enzyme purification included homogenization, extraction of pigments followed by consecutive chromatographies on DEAE-Sepharose and Superdex 200.
The purification factor for purified date palm peroxidase was 17 with 5.8 % yield.
The purity was checked by SDS and native PAGE, which showed a single prominent band.
The molecular weight of the enzyme was approximately 55 kDa as estimated by SDS–PAGE.
The enzyme was characterized for thermal and pH stability, and kinetic parameters were determined using guaiacol as substrate.
The optimum activity was between pH 5–6.
The enzyme showed maximum activity at 55 °C and was fairly stable up to 75 °C, with 42 % loss of activity.
Date palm leaves peroxidase showed Km values of 0.77 and 0.045 mM for guaiacol and H2O2, respectively.
These properties suggest that this enzyme could be a promising tool for applications in different analytical determinations as well as for treatment of industrial effluents at low cost.
American Psychological Association (APA)
al-Sunaydi, Abd al-Rahman M.& Ismail, Muhammad A.. 2011. Purification and characterization of membrane-bound peroxidase from date palm leaves (Phoenix dactylifera L.). Saudi Journal of Biological Sciences،Vol. 18, no. 3, pp.293-298.
https://search.emarefa.net/detail/BIM-364456
Modern Language Association (MLA)
al-Sunaydi, Abd al-Rahman M.& Ismail, Muhammad A.. Purification and characterization of membrane-bound peroxidase from date palm leaves (Phoenix dactylifera L.). Saudi Journal of Biological Sciences Vol. 18, no. 3 (2011), pp.293-298.
https://search.emarefa.net/detail/BIM-364456
American Medical Association (AMA)
al-Sunaydi, Abd al-Rahman M.& Ismail, Muhammad A.. Purification and characterization of membrane-bound peroxidase from date palm leaves (Phoenix dactylifera L.). Saudi Journal of Biological Sciences. 2011. Vol. 18, no. 3, pp.293-298.
https://search.emarefa.net/detail/BIM-364456
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 298
Record ID
BIM-364456