Phytochemical screening and biological activities of striga hermonthica and Nigella sativa (seeds)
Dissertant
Mahmud, Yasamin Hasan al-Shaykh
Thesis advisor
Abd al-Majid, Muna Abd al-Munim Muhammad
University
Omdurman Islamic University
Faculty
Faculty of Pharmacy
Department
Department of Pharmacognosy
University Country
Sudan
Degree
Master
Degree Date
2012
English Abstract
Medicinal plants constitute are an effective source of both traditional and modern medicines, herbal medicine has been shown to have genuine utility.
80% of rural population depends on natural products as primary health care (WHO, 1978).
Striga hermonthica (Scrophulariaceae) and Nigella sativa seeds (Ranunculaceae) are widely used in the different areas of Sudan for treatment of various diseases.
Each plant was extracted in a soxhlet with n-hexane, ethyl acetate and 70 % ethanol successively.
Also the whole plant of Striga hermonthica was extracted by maceration with glacial acetic acid and its ash was extracted with distilled water.
The chemical constituents of each plant were identified.
The results of S.
hermonthica and Nigella sativa seeds exhibited presence of flavonoid, tannin, steroid and triterpine, saponin, alkaloid, cardiac glycoside and reducing compound.
The antimicrobial activity of all extracts of each plant were tested against four standard strains of bacteria : Staphylococcus aureus , Bacillus stearothermophilus, Escherichia coli , Pseudomonas aeruginosa and two standard strain of fungi : Candida albicans, Aspergillus niger.
A maximum antibacterial activity was observed by the Glacial acetic acid extract of Striga hermonthica while the ethanol extract of whole plant reflected moderate activity.
All extracts showed no activity against all fungal strains tested.
The ethanol extract of Nigella sativa seeds showed a moderate activity against bacteria strains tested.
HPLC analysis for Striga hermonthica glacial acetic acid extract showed the presence of phenolic compound like gallic acid, chlorogenic acid, resorcinol, catechin ,p-hydroxy benzoic acid, luteolin -3- methoxy -7- rutinoside, vanillin, rutin, phenol, p- coumaric acid, luteoline, quercetin, salicylic acid, apigenin.
The antitumor activity was investigated using different cell lines as : HEPG2, MCF7, and HEP2.
Striga hermonthica ash extract exhibited high activity against liver carcinoma used compared with standard drug used.
The cytotoxicity of glacial acetic acid extract at 139 ppm exhibited LC50 equivalent to 27.
Main Subjects
No. of Pages
104
Table of Contents
Table of contents.
Abstract.
Chapter One : Introduction.
Chapter Two : Experimental Materials and methods.
Chapter Three : Results and discussion.
Recommendations and conclusion.
References.
American Psychological Association (APA)
Mahmud, Yasamin Hasan al-Shaykh. (2012). Phytochemical screening and biological activities of striga hermonthica and Nigella sativa (seeds). (Master's theses Theses and Dissertations Master). Omdurman Islamic University, Sudan
https://search.emarefa.net/detail/BIM-364848
Modern Language Association (MLA)
Mahmud, Yasamin Hasan al-Shaykh. Phytochemical screening and biological activities of striga hermonthica and Nigella sativa (seeds). (Master's theses Theses and Dissertations Master). Omdurman Islamic University. (2012).
https://search.emarefa.net/detail/BIM-364848
American Medical Association (AMA)
Mahmud, Yasamin Hasan al-Shaykh. (2012). Phytochemical screening and biological activities of striga hermonthica and Nigella sativa (seeds). (Master's theses Theses and Dissertations Master). Omdurman Islamic University, Sudan
https://search.emarefa.net/detail/BIM-364848
Language
English
Data Type
Arab Theses
Record ID
BIM-364848
