Antimicrobial and wound healing activity of Khaya senegalensis and Dichrostachys cinerea against standard and clinical isolates
Dissertant
Thesis advisor
al-Maqbul, Aishah Zuhayr
Umar, Muhammad al-Fatih Ahmad
University
Omdurman Islamic University
Faculty
Faculty of Medical Laboratory
Department
Department of Medical Microbiology
University Country
Sudan
Degree
Master
Degree Date
2009
English Abstract
In this study twelve leaves and bark extracts of two Sudanese medicinal plants (Khaya senegalensis and Dichrostachys cinerea) were screened for their antimicrobial activity against two Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus), three Gram negative bacteria (Escherichia coli, Proteus vulgaris and Pseudomonas aeruginosa) and two fungi (Aspergillus niger and Candida albicans).
Most of the extracts exhibited inhibitory activity against one or more of the tested organisms.
Six extracts showed inhibitory effects to the two Gram positive tested organisms.
Bacillus subtilis and Staphylococcus aureus were the most susceptible organisms to the extracts while Proteus vulgaris showed the lowest susceptibility.
Four extracts showed inhibitory effects to the two fungi tested ; Aspergillus niger showed the lowest susceptibility while the Candida albicans was the most susceptible organism.
The minimum inhibitory concentrations (MIC) of the methanolic extracts of the Khaya senegalensis against standard organisms were determined using the agar plate dilution method.
The activity of four reference antibiotics were determined against the five tested standard bacteria and compared to the activity of the tested plant extracts.
The antifungal activity of two reference drugs was determined against the two tested fungi and compared to antifungal activity of` the tested plant extracts.
The most active plant extracts were tested against 100 clinical isolates colleted randomly from National Health Laboratory, Khartoum Teaching Hospital, Police Teaching Hospital and Khartoum North Teaching Hospital.
In study, the wound healing effect of Khaya senegalensis bark extract was investigated on open skin in rats.
Thirty albino rats of either sex weighting (80 - 100) gm were used during the study.
Hairs of the back and right flank of animal were completely shaved and full-thickness a circular wound was made on the shaved area.
Methanolic extract of Khaya senegalensis bark was prepared.
Ointment of 1%, 2% and 5 % (w/w) extract polyethylene glycol were prepared.
The ointment extracts were applied twice a day.
Tetracycline ointment was used as standard control.
Two trials were performed, the first using groups non-infected rats and the second using groups artificially infected rats with Staphylococcus aureus.
Treated groups were compared with non-treated groups.
Healing was determined by measuring the reduction in wound area.
The results confirmed that Khaya senegalensis ointment is a potent healing agent even better than Tetracycline ointment at the concentration used.
Main Subjects
Topics
No. of Pages
114
Table of Contents
Table of contents.
Abstract.
Chapter One : Introduction and literature review.
Chapter Two : Materials and methods.
Chapter Three : Result and discussion.
Conclusions and recommendations.
References.
American Psychological Association (APA)
Yaqub, Hasan Yaqub Abd Allah. (2009). Antimicrobial and wound healing activity of Khaya senegalensis and Dichrostachys cinerea against standard and clinical isolates. (Master's theses Theses and Dissertations Master). Omdurman Islamic University, Sudan
https://search.emarefa.net/detail/BIM-365029
Modern Language Association (MLA)
Yaqub, Hasan Yaqub Abd Allah. Antimicrobial and wound healing activity of Khaya senegalensis and Dichrostachys cinerea against standard and clinical isolates. (Master's theses Theses and Dissertations Master). Omdurman Islamic University. (2009).
https://search.emarefa.net/detail/BIM-365029
American Medical Association (AMA)
Yaqub, Hasan Yaqub Abd Allah. (2009). Antimicrobial and wound healing activity of Khaya senegalensis and Dichrostachys cinerea against standard and clinical isolates. (Master's theses Theses and Dissertations Master). Omdurman Islamic University, Sudan
https://search.emarefa.net/detail/BIM-365029
Language
English
Data Type
Arab Theses
Record ID
BIM-365029
