Solubilization and partial purification of the plasma membrane ATpase of Rhodotorula glutinis
Other Title(s)
الإذابة و التنقية الجزيئة لإنزيم الأتيبيز من الغشاء البلازمي لخميرة الرودوتيلا جليتينس
Joint Authors
Qumsani, Taha Abd Allah
Griffin, Charles C.
Source
Journal of King Abdulaziz University : Sciences
Issue
Vol. 17, Issue 1 (31 Dec. 2005), pp.153-166, 14 p.
Publisher
King Abdulaziz University Scientific Publishing Center
Publication Date
2005-12-31
Country of Publication
Saudi Arabia
No. of Pages
14
Main Subjects
Abstract EN
The optimal conditions for solubilization of the ATPase activity from the plasma membrane of Rhodotorula glutinis by zwitterionic detergent Z-3-14 were found to be: 0.55 mg/ml (5X cmc) of Z- 3-14, at room temperature in the presence of 1 mM ATP, 40% (v/v) glycerol, 11.
25 % ammonium sulfate, 5 mM 2- mercaptoethanol, 1 mM PMSF in 0.
1 M Mes-Tris pH 6.5, followed by sonication in a water bath sonicator for 6 min.
A 4-fold purification of the solubilized ATPase activity was obtained by centrifugation of the Z-3-14 extract through a glycerol gradient (20-50 %, v / v).
American Psychological Association (APA)
Qumsani, Taha Abd Allah& Griffin, Charles C.. 2005. Solubilization and partial purification of the plasma membrane ATpase of Rhodotorula glutinis. Journal of King Abdulaziz University : Sciences،Vol. 17, no. 1, pp.153-166.
https://search.emarefa.net/detail/BIM-365118
Modern Language Association (MLA)
Qumsani, Taha Abd Allah& Griffin, Charles C.. Solubilization and partial purification of the plasma membrane ATpase of Rhodotorula glutinis. Journal of King Abdulaziz University : Sciences Vol. 17, no. 1 ( 2005), pp.153-166.
https://search.emarefa.net/detail/BIM-365118
American Medical Association (AMA)
Qumsani, Taha Abd Allah& Griffin, Charles C.. Solubilization and partial purification of the plasma membrane ATpase of Rhodotorula glutinis. Journal of King Abdulaziz University : Sciences. 2005. Vol. 17, no. 1, pp.153-166.
https://search.emarefa.net/detail/BIM-365118
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 164-165
Record ID
BIM-365118