Lucigenin-and luminol-enhanced chemiluminescence in mechanical injured roots of corn (Zea mays L.)‎

Abstract EN

The chemiluminescence (CL) of roots from com (Zea mays L.) seedling , after mechanical injury , has been observed by lucigenin - or luminol dependent chemiluminescence (LCDC) and ( LMDC) respectively.

The light emission in injured roots system was blocked by NaCN m LMDC system whereas ascorbic acid addition resulted about 50/o an o reduction in light emission in LMDC-and LCDC systems respectively.

In another experiment of injured roots extracts, the Light emission was increased by the addition of H202 in LCDC and LMDC systems, whereas NaCN addition has blocked effect in (LMDC) system.

The suggested results in this study shows the effect of CL substrates , such as luminol or lucigenin and the addition compounds (NaCN or ascorbic acid radical scavenger ) on the CL intensity , may be proved the participation of reactive oxygen species, mainly superoxide anion , and t e free radical mechanism in the CL - injured roots reaction of com plant seedlings