تقدير كميات مايكروية من الدواء Spironolactone في بعض المستحضرات الصيدلانية باستخدام تقنية التألق الجزيئي

Abstract EN

The present study include a new developed method of analysis for determination of drug Spironolaction (SP) in some Pharmaceuticals by Spectrofluorometric method.

Spironolaction was determined under optimal experimental condition that follows :-The excitation spectrum was (^=351 nm), the emmetion spectrum was (^=518 nm), pH=1, the suitable temperature for reaction 60oC and the optimal time less than (3) minute.

The analysis and rang statistical data was:-Linear dynamic rang (1-10) ^g.ml-1, the detection limit (D.L = 0.023 ^g.ml-1), Molar absorptivity (s = 29875 liter mole-1 cm-1), Relative standard deviation (%RSD = 0.78), (%Erel = 3.3) and recovery (Rec = 96.6) percentage.

Determination of Spironolactone was accomplished by two methods (A) Using direct calibration curve method, Corr.

Coef.

(R = 0.988 and %Rec = 97.4).

(B) Using standard additional methods Corr.

Coef (R =0.988 and %Rec= 96.6).