Myotoxic and hyperlipidemic effects of diazinon in female rats

Abstract EN

-Background : Diazinon (DZ) is used in ectoparasiticide formulations and for external parasitic control, resulting in distribution of diazinon in the with environment deleterious effects on biological systems.

Therefore, the effect of different doses of diazinon on serum biochemical parameters was tested in our study.

Material and Methods : Thirty five female Sprague-Dawely rats (180- 220g) were divided into 7 females as controls and received 0.

2 ml saline by gavage and 28 female rats (diazinon- treated group) orally received 8, 10, 12 and 20 mg / Kg body weight of diazinon for 3 weeks.

Results showed that treatment with Dz induced significant (p < 0.

05) increase in the level of serum malondialdehyde (MDA) and the activity of lactate dehydrogenase (LDH) in the diazinon- treated groups compared to the control group.

On the other hand, the results revealed a significant (p < 0.

05) decrease in the activities of serum acetylcholinestrase (AChE), glutathione peroxidase (GPx) and superoxide dismutase (SOD) with varying degrees, according to the effect of different doses of DZ in the diazinon-treated groups, compared to the control.

Meanwhile, the results showed that treatment of rats with different doses of diazinon induced significant (p < 0.

05) increase in serum total lipids, total cholesterol, triglycerides, high density lipoprotein (HDL-C) and low density lipoprotein (LDL-C) compared to the control group.

The histological analysis of heart tissues demonstrated different degrees of degeneration according to the dose of Dz.

Also, large areas of degenerating muscle fibers were pale stained with evident loss of transverse striations, and wide interfascicular spaces were shown in diazinon-treated rats with different degrees of degenerating muscle fibers, varied with the dose of diazinon.

Conclusions : These results suggest that oral administration of diazinon causes muscular toxicity.

However, the myotoxicity might be due to elevated concentrations of free oxygen radicals released upon diazinon exposure, indicating alterations in