Gel clot assay used for detection of Candida spp. infection in urinary tract

Abstract EN

The study including isolation and identification of Candida spp.

causing UTIs from patients coming to Al- Yarmouk hospital.

A local diagnostic kit was used for measuring canditoxin activity in the urine of patients and it’s relation to Candida spp.

50 urine samples were collected from female patients of different ages with UTI.

The percentage of C.

albicans was (26%); C.

tropicalis was (16%).

The results showed a high significant difference between them (p=0.00, chi=21.0).

The study comprised and attempts to prepare a local diagnostic kit from the lysate of Homarus lobster, used to indicate the presence of canditoxin and measure it’s activity also in urine samples.

The study showed a significant increase in canditoxin activity in UTI patients, (69.2%) for C.

albicans, (75%) for C.

tropicalis while the percentage of the patients who have no fungal growth was 17.24%.

The results showed that the percentage of positive LAL assay in-patients diagnosed with fungal infection was (40%) as compared with negative results (60%).