Determination of iron in human serum samples of thalassaemia patients by flame atomic absorption

Abstract EN

A simple and sensitive flame atomic absorption spectrophotometricmethod has been used for the determination of iron concentration in blood serum for both (male and female) of Thalassemia patients group and normal group.

The method is compared with a standard spectrophotometric method.

The two methods are based on the reaction of iron with ferene reagent at acidic condition.

The results of 84 samples showed that the distribution of iron concentration between serum samples is ranged between (401.3) and (440.3) μg / dL for female and male respectively of thalassemia patients group, (66.3),(90.6) μg / dL for normal female and male group, so the analysis of iron levels in serum showed that iron levels in serum of thalassemia patients group were significantly higher than the normal group and the value of iron in the serum of the male of thalassemia patients group and normal group is higher than female.

Values of iron content measured in the serum samples by flame atomic absorption method are in good agreement with the results obtained with the standard spectrophotometric method, the calculated t value was less than t tabulated value, therefore no significant difference between the two methods at 95 % confidence level was found.