In vitro age-dependent leukocytes within whole blood activity among healthy adult men in Basrah measured by chemiluminescence

Abstract EN

All immune cells are affected by aging, a fact contributes to the high susceptibility to infections and increased morbidity and mortality observed in the elderly.

Non-isolated granulocytes were tested for their phagocytic activity in whole blood in vitro to mirror the in vivo stimulation of granulocytes and the effect of age progress on their phagocytic activity.

Blood samples were obtained from (420) healthy adult men with different age groups from (16-60) year.

These samples were prepared for chemiluminescence (CL) measurements.

Luminol was used to increase the amount of measurable light emitted due to liberation of oxygen metabolites during phagocytosis.

Chemiluminescence was continuously recorded on a chart recorder, and all the measurements were estimated in mm peak height and related to the same number of cells i.e.

(100 cell), for the purpose of the comparison between age groups.

In this study a significant difference was demonstrated (P < 0.05) between leukocytes phagocytic activity with different age groups.