Molecular and serological identification of foot-and-mouth disease virus serotypes in cattle of Basrah Province

Abstract EN

This study was conducted on serum from 6 different regions covering all parts of the Basrah province during the period from October 2012 to June 2013 in order to determine the prevalence of foot and mouth disease in bovine species.

From the serology results, the overall prevalence of this disease was (72.7 %).

Of the 165 samples subjected to Nonstructural protein (NSP) enzyme linked immunosorbent assay (ELISA) screening test, 120 were interpreted as positive representing (72.7 %) while the other 45 samples were negative representing 27.3 %.

The seropositivity significantly associated with age groups (p < 0.01) and sex (P < 0.05).

RT-PCR base detection of FMDV for primary and serotype specific diagnosis was used.

Different types of clinically positive samples of FMD were collected from the cattle which includes samples from mouth vesicles, serum and saliva.

RT-PCR based identification was done with (universal primer sets 1F / 1R, A- 1C562, O-1C272 and As1-1C505) with expected band of (328, 866, 639 and 911 bps.

respectively).

Of eighty three samples collected, only 68(81.9 %) were successfully amplified by RT-PCR, and of these, only 19(22.9 %) samples were found to be serotype A-1C562 positive and 26(31.3 %) were serotype O-1C272 positive and nil for serotype As1-1C505.

The frequency of foot-and-mouth disease viral genome presence in cattle had no significant differences based on sex (P > 0.05) while the difference among age groups was significant (P < 0.05).