Pronase-free B-cell flow-cytometry crossmatch
Joint Authors
Hajeer, Ali H.
Salih, S.
Sutton, Paul
Shubaili, A.
Anazi, H.
Source
Saudi Journal of Kidney Diseases and Transplantation
Issue
Vol. 20, Issue 4 (31 Aug. 2009), pp.662-665, 4 p.
Publisher
Saudi Center for Organ Transplantation
Publication Date
2009-08-31
Country of Publication
Saudi Arabia
No. of Pages
4
Main Subjects
Topics
Abstract EN
Detection of anti-class II antibodies by panel response assay (PRA) and flow crossmatch techniques carries an important value in terms of graft function.
Even low levels of preformed alloantibodies to HLA class II antigens represent a risk of rejection.
We present here a method for blocking non-specific flow crossmatch reactions using pooled, heat-inactivated rabbit serum.
This method shows very low background and minimal non-specific reactions.
In addition, it avoids the use pronase enzyme that can non-specifically digest different cell surface proteins.
American Psychological Association (APA)
Hajeer, Ali H.& Salih, S.& Sutton, Paul& Shubaili, A.& Anazi, H.. 2009. Pronase-free B-cell flow-cytometry crossmatch. Saudi Journal of Kidney Diseases and Transplantation،Vol. 20, no. 4, pp.662-665.
https://search.emarefa.net/detail/BIM-37341
Modern Language Association (MLA)
Hajeer, Ali H.…[et al.]. Pronase-free B-cell flow-cytometry crossmatch. Saudi Journal of Kidney Diseases and Transplantation Vol. 20, no. 4 (Dec. 2009), pp.662-665.
https://search.emarefa.net/detail/BIM-37341
American Medical Association (AMA)
Hajeer, Ali H.& Salih, S.& Sutton, Paul& Shubaili, A.& Anazi, H.. Pronase-free B-cell flow-cytometry crossmatch. Saudi Journal of Kidney Diseases and Transplantation. 2009. Vol. 20, no. 4, pp.662-665.
https://search.emarefa.net/detail/BIM-37341
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 664-665
Record ID
BIM-37341
