Preparation of vaccine against diabetic foot pathogenic bacteria using low level diode laser
Joint Authors
Radi, Zaynab Awwad
Rustum, Ihsan F.
al-Mukhtar, Sadiq Abbas
Source
al-Kindy College Medical Journal
Issue
Vol. 9, Issue 2 (31 Dec. 2013), pp.29-31, 3 p.
Publisher
University of Baghdad al-Kindi College of Medicine
Publication Date
2013-12-31
Country of Publication
Iraq
No. of Pages
3
Main Subjects
Topics
Abstract EN
Backgrround:: Since the invention of laser in 1960, lasers have been developed and approved in many fields.
Lasers can now be regarded as practical tools with unique properties that have been utilized effectively in several applications in fields of medical and biological sciences.
Objjecttiives:: The aim of the current study was to preparation of vaccines (live attenuated and killed) by irradiation of the bacteria by the low level diode laser.
Metthods:: six bacterial isolates were isolated from human samples of diabetic foot infections, which used for preparation of vaccines.
The experiment was conducted on fifteen adult male rabbits; they were divided into three groups with 5 rabbits each.
Blood samples were collected from the marginal ear vein of the rabbits after one month of the vaccination, for the purpose of measuring the concentration of the immunoglobulins which present in their serum, using Radial Immunodiffusion (RID) method by specialized kits (LTA-Italy).
Resulltts:: After irradiation of the bacterial suspensions with the diode laser for different exposure times and different frequencies, and the wavelengths used were (660, 820, 915) nm, the growth of bacterial isolates decreased until killed of bacteria at 40 min.
The results of IgA concentrations for the three groups were highly significant (P < 0.01) when comparing the attenuated with control group, while were significant (P < 0.05) between the killed vaccine and control groups, and not significant variations between the attenuated and killed groups, Concllusiions:: Wavelength (660) nm was more effective in killing the bacteria, and the variations were not significant between the live attenuated and the killed vaccine
American Psychological Association (APA)
Radi, Zaynab Awwad& Rustum, Ihsan F.& al-Mukhtar, Sadiq Abbas. 2013. Preparation of vaccine against diabetic foot pathogenic bacteria using low level diode laser. al-Kindy College Medical Journal،Vol. 9, no. 2, pp.29-31.
https://search.emarefa.net/detail/BIM-374186
Modern Language Association (MLA)
Radi, Zaynab Awwad…[et al.]. Preparation of vaccine against diabetic foot pathogenic bacteria using low level diode laser. al-Kindy College Medical Journal Vol. 9, no. 2 (2013), pp.29-31.
https://search.emarefa.net/detail/BIM-374186
American Medical Association (AMA)
Radi, Zaynab Awwad& Rustum, Ihsan F.& al-Mukhtar, Sadiq Abbas. Preparation of vaccine against diabetic foot pathogenic bacteria using low level diode laser. al-Kindy College Medical Journal. 2013. Vol. 9, no. 2, pp.29-31.
https://search.emarefa.net/detail/BIM-374186
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 30-31
Record ID
BIM-374186