Antimicrobial effects of allium sativum linn. and eugenia caryophyllus extracts against bacteria isolates from clinical samples

Abstract AR

Crude extracts from dried garlic (Allium sativum) and cloves (Eugenia caryophyllus) were studied for their antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa..

A total 60 clinical specimens were collected from patients clinically diagnosed as cases of bacterial infections in Marghan Hospital in Babylon.

Gram positive bacteria were isolated from 43 specimens and 17 specimens were gram negative bacteria.

Gram positive bacteria identified by cultural and biochemical tests , among those isolates , 24 isolates were Staphylococcus epidermidis and 19 isolates were Staphylococcus aureus .

Gram negative bacteria identified by cultural and biochemical tests and all isolates were Pseudomonas aeruginosa .

The effect of ethanolic and water extract were tested against isolating bacteria by using agar well diffusion method.

Forty microliters of the dilution of Crude extracts was transferred to each well and sterile water served as a control.

Extract from cloves showed the highest activity with minimum inhibitory concentration (MIC) ranging from 100 to 400 mg/ml, .Garlic extract showed no inhibitory effect against these bacteria.

The inhibitory activity of the herbs extracts decreased over the storage period 4 weeks at 4c◦.