Molecular study of virulence genes of brucella isolated from human clinical cases in Babylon province

Abstract EN

The study was carried out in seven medical region; Babylon Hospital for Maternal and Pediatrics, Al-Hilla Surgical Teaching Hospital, Al- Qasim general Hospital, Al- Hashimia general Hospital, Marjan Hospital, Babylon public health libratory, and private labs.

During the period from Dec.- 2010 to Jan.-2012.

To evaluate the results of Molecular study of verulance factors gene of Brucellosis in human population.

A total No.

of 340 clinical blood samples5 ml were obtained from suspected patients with Brucellosis positive RBPT, Thirty from both sexes apparently healthy human individuals with rose Bengal negative were included as a control in this study.

The samples were taken before adequate antibiotic treatment with age, sex group ranged from 9-70 years.

Study the virulence factors of Brucella species.

Is important at evaluated the Pathogenicity of this bacteria.

Eight virulence factors were detected by using PCR technique, that all Brucella isolates possessed, Detection of virulence factors of Brucella isolates in present study , incidence of specific PCR amplified fragment for genes (CBG, mviN ,manA, manB, wbkA, omp25, omp31, and znuA).

The virulence genes was present in most isolates belonging to Brucella spp.

It was done at multiplex PCR by using two genes in each process, no.1, 2, 3, 6, 7, 8 belong to B.

melitensis, while no.

4, 5 to B.

abortus.

These results indicate that each steps in the Brucella infection process can be mediated by a number of virulence factors and each strain may have a unique combination of these factors, this collection of virulence genes is made possible by the variety of genetic factors contributing the genome plasticity, such as plasmid, phages, and transposon elements, the total lack of virulence factor ratio (37.5 %) in B.abortus than B.

melitensis (12.5 %) and the loses of this genes of total isolates were (50 %)..

It was significant differences at level (P ≤ o.o5).