![](/images/graphics-bg.png)
Isolation and identification of Saccharomyces cerevisiae var boulardii and its uses as a probiotic (in vitro)
Other Title(s)
عزل و تشخيص خميرة Saccharomyces cerevisiae var. boulardii و استعمالها أحياءا علاجية في المختبر (in vitro)
Joint Authors
Khidr, Khanda O.
al-Zubaydi, Zaynab M.
Source
Issue
Vol. 25, Issue 1 (31 Mar. 2014), pp.1-11, 11 p.
Publisher
University of Mosul College of Science
Publication Date
2014-03-31
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Topics
Abstract AR
تضمنت الدراسة عزل و تشخيص Saccharomyces cerevisiae var.
boulardii من الفواكه الطازجة و المجففة مثل (العنب و التين و المشمش و المانغوستين و اللايتشي) المتوفرة في الأسواق المحلية في مدينة اربيل.
و تم الحصول على 50 عزلة من خميرة Saccharomyces cerevisiae var.
boulardii اعتمادا على الصفات الشكلية و الكيموحيوية و الفسلجية، كما اختبرت كفاءتها التثبيطية بكتيريا Salmonella enteric sub spp.
enterica (Typhimurium) بطريقة الانتشار في الحفر .(Well Diffusion) معظم العزلات أظهرت فعالية تثبيطية ضد البكتيريا المختبرة، و أعلى قطر منطقة كانت (16 ملم) ضد S.Typhimurium للعزلة (S.b32)عشرة عزلات أظهرت أعلى فعالية تثبيطية استعملت لدراسة صفاتها الفسلجية مثل تحملها لتراكيز مختلفة من أملاح الصفراء و الحوامض المعوية و هذه العزلات هي (S.b6, S.c1, S.b2, S.c36, S.b13, S.b28, S.b32, S.b22, S.b48, and S.
b5).
و من خلال دراسة الصفات الفسلجية لعزلات الخمائر لوحظ أن العزلة (S.b32) كانت اكر مقاومة من العزلات الأخرى لتراكيز مختلفة من أملاح الصفراء و الحوامض المعوية.
Abstract EN
The study included isolation and identification of Saccharomyces cerevisiae var.
boulardii from fresh and dried fruits such as (fig, grape, apricot, lychees, mangosteen) which were available in local markets in Erbil city.
Fifty isolates of the yeast were obtained depending on morphological, physiological and biochemical tests.
The antibacterial activity of the isolated yeasts against pathogenic bacteria Salmonella enteric sub spp.
enterica (Typhimurium) in vitro was determined by Well's Diffusion method.
Most of the isolated yeasts showed antibacterial activity against tested bacterium, the highest diameter of inhibition zone was (16 mm) for isolates (S.b32).
The ability to tolerate different concentrations of bile salt and gastric acids were also studied.
Ten strains of the isolated yeast had the highest inhibitory activity and were used to study physiological properties (bile salt concentration and gastric acidity) which were (S.b6, S.c1, S.b2, S.c36, S.b13, S.b28, S.b32, S.b22, S.b48, and S.b5).
The result showed that the isolate (S.b32) was more resistant than other strains when exposed to bile salt and gastric acids.
American Psychological Association (APA)
al-Zubaydi, Zaynab M.& Khidr, Khanda O.. 2014. Isolation and identification of Saccharomyces cerevisiae var boulardii and its uses as a probiotic (in vitro). Rafidain Journal of Science،Vol. 25, no. 1, pp.1-11.
https://search.emarefa.net/detail/BIM-374739
Modern Language Association (MLA)
al-Zubaydi, Zaynab M.& Khidr, Khanda O.. Isolation and identification of Saccharomyces cerevisiae var boulardii and its uses as a probiotic (in vitro). Rafidain Journal of Science Vol. 25, no. 1 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-374739
American Medical Association (AMA)
al-Zubaydi, Zaynab M.& Khidr, Khanda O.. Isolation and identification of Saccharomyces cerevisiae var boulardii and its uses as a probiotic (in vitro). Rafidain Journal of Science. 2014. Vol. 25, no. 1, pp.1-11.
https://search.emarefa.net/detail/BIM-374739
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 9-11
Record ID
BIM-374739