استخدام اختبار إليزا للكشف السريع عن فيروس البطاطا واي (Potato virus Y)‎ في حقول البطاطا في العراق

Abstract EN

This study was conducted to evaluate the use of enzyme-linked immounosorbent assay (ELISA) for rapid detection of potato virus Y (PVY) in potato fields, particularly seed potato fields, in Iraq.

The virus was isolated from naturally infected potato plants and identified according to symptoms produced on diagnostic hosts, host range, sap stability and serological test.

The virus was purified and used for antiserum production.

Results showed that ELISA can detect PVY rapidly even at low concentration in purified preparation as well as in sap of infected plants.

The application of ELISA was used sucessufully for detecting PVY in crude sap of infected plants collected from potato fields.

The use of ELISA test can be adopted to facilitate the work of the national seed potato program through its practical applicability for testing basic stocks and for assessing the suitability of field – grown materials for use in the following season and as a tool for certification schemes.

The technique was very sensitive and can be used for processing a large number of samples in a very short time