The effects of cigarette smoke on some hematological parameters in human

Abstract EN

The effects of cigarette smoke on Red Blood Cells (RBCs) membranes were studied.

Spontaneous hemolysis test indicated that RBCs which were separated from heavier smokers’ blood (more than 20 cigarettes/day) have higher percentage of hemolysis when compared to those separated from nonsmokers blood(15±0.7%, 1.8±0.2%, respectively).

The results of osmotic fragility test for the above RBCs from both volunteers indicate, a slight shift to the left(MCF(50%)=4.4+0.05,4.55±0.06 g/1 , respectively), this illustrate that the resistance to lysis was increased( the volume to the surface area ratio was decreased).

In the analysis of phospholipid’s composition of RBCs, we observed that the percentage of diphosphoglyceride(DPG) and lysophosphatidylcholine (LPC) to the total phospholipids were increased for cigarette smokers.

Also results obtained from measuring the superoxide radicals in smokers’ blood have high optical density than that for nonsmokers, also for conjugated dienes.

Our results indicate that the cigarette smoke has many effects on RBCs specially on their membranes and phospholipid’s composition, and these effects may be due to cigarette smoke contents and free radicals.