Anaerobic degradation of crude oil by sulphate reducing bacteria isolated from soils contaminated with etroleum hydrocarbons

Abstract EN

This study included isolatation a mix cultures of sulphate reducing bacteria from soils in Shaeba regain contaminated with petroleum hydrocarbons by using API medium enriched with oxygen reducing agents and saturated with gas (90% N2 ) and ( 10% CO2 ) by using sodium lactate as sole carbon source.

The result showed that the number of bacteria ranged ( 4 x 105 – 4.5 x 105 ) cells / g of soil.

Also using crude oil as a sole of carbon source to study the ability of sulphate reducing bacteria on degradation of crude oil under anaerobic condition .

The result showed these bacteria have high ability for degradation of crude oil.

The percent rate of degradation in culture was ( 84.4 % ) after (54) days incubation and the rate concentration of total petroleum hydrocarbons ( TPH ) extraction from culture value ( 22.06 ) µg / L , while in control was ( 190.84 ) µg / L