Effect of 3,5-diiodothyropropionic acid (DITPA)‎ on renal ischemia-reperfusion Injury in a Mouse model

Abstract EN

Introduction : Acute renal failure (ARF) is an important clinical problem with a high mortality and morbidity.

Ischemia/reperfusion (I / R) was considered one of the primary causes of ARF.

Inflammatory process is thought to be the major mechanism that contribute to I / R injury.

There are evidences of the importance of thyroid hormone (TH) in the response of the myocardium to ischemic stress and cardiac remodeling following myocardial infarction.

3,5-diiodothyropropionic acid (DITPA) is a synthetic TH analogue with a low metabolic activity.

Also DITPA have evidences of improving effects on I / R in heart through modulation of cellular signaling in response to ischemic stress and this modulation may be beneficial in protection from renal I / R damage.

Objectives : The objective of present study was to assess the effects of DITPA on renal I/R injury and the resulted kidney dysfunction in a mouse model.

Materials and Methods : A total of 18 Adult males of Swiss albino mice were randomized to three groups : I / R group (n = 6), mice underwent 30 minute bilateral renal ischemia and 48 hr reperfusion.

Sham group (n = 6), mice underwent same anesthetic and surgical procedures except for ischemia induction.

DITPA treated group : (n = 6), I / R + DITPA (3.75 mg / kg) by intraperitoneal injection.

After the end of reperfusion phase mice were sacrificed, blood samples were collected directly from the heart for determination of serum IL-18, IL-6, urea and Creatinine.

Both kidney were excised, the right one homogenized for oxidative stress parameters (MDA and GSH) measurements and the left kidney fixed in formalin for histological examination.

Results : Serum IL-18, IL-6, urea and Creatinine, kidney MDA levels and scores of histopathological changes were significantly (P < 0.05) elevated in I / R group as compared with that of sham group.

Kidney GSH level was significantly (P < 0.05) decreased in I / R group as compared with that of sham group.

DITPA caused nonsignificant (P > 0.05) changes in levels of all study parameters as compared with that of I / R group.

Conclusion : The results of the present study show that DITPA insignificantly ameliorated kidney damage resulted from I / R.