Isolation of Pseudomonas aeruginosa from clinical cases and environmental samples, and analysis of its antibiotic resistant spectrum at Hilla Teaching Hospital

Abstract EN

The present study included 152 samples collected from different clinical cases and environmental samples in Hilla General Teaching Hospital during the period from October 2010 to January 2011.

The age of the patients ranged from (6 to 65) years.

The samples were immediately inoculated on pseudomonas isolation agar plates.

All plates were incubated aerobically at 37ºC for 24-48 hrs.

Results of morphological and biochemical characterization tests revealed that only 48 (31.57 %) isolates were Ps.

aeruginosa, while other 104 (68.43 %) represented other bacterial genera.

Antibiotic susceptibility test for all the isolates was determined by using disc diffusion test (DDT) and rapid iodiometric methods.

The results showed that, the Ps.

aeruginosa isolates were fully resistant (100 %) for each the following antibiotics ; ampicillin, Cefotaxime, chloramphenicol, penicillin, doxycycline, and Erythromycin, while they exhibited moderate resistance to Amikacin 19 (39.5 %), ciprofloxacin 15 (31.26 %) and Polymyxine 29 (40 %), and were sensitive to Pipracillin, Ticarciline in a percentage rate (20.08 %) for each antibiotic.

The bacterium was found to carry beta lactamase enzymes with high rate of resistance (66.6 %).