Estimation of humural immune response on the rabbits that immunizing with hydatid cyst antigens by using IHAT and ElISA
Joint Authors
Idan, Ikhlas Mushrif
Ardalan, Nakshin Mirza Mahmud
Source
Journal of the Faculty of Medicine Baghdad
Issue
Vol. 51, Issue 3 (30 Sep. 2009), pp.332-335, 4 p.
Publisher
University of Baghdad Faculty of Medicine
Publication Date
2009-09-30
Country of Publication
Iraq
No. of Pages
4
Main Subjects
Abstract EN
Background : Hydatid disease also known as echinococcosis or hydatidosis , is caused by infection with larva (metacestoide) of tape worms of the genus Echinococcus.
Materials and method : Twelve Rabbits were immunized with three types of antigens (Hydatid cyst fluid antigen; Protoscoleces antigen and Germinal and Laminated layer antigen) according to a specific immunization program and we used indirect hemagglutination test( IHAT) and Enzyme Linked Immuno Sorbent Assay(ELISA) for detecting the anti-Echinococcus antigens in the serum of these animals.
Results : The highest titter of antibodies were recorded in the Rabbit serum when use the first antigen (Hydatid cyst fluid) by using IHA method (1 : 64)after the end of immunization program while in ELISA method reached (1 : 128), The serum level for the rabbit serum that immunization with the second antigen(Protoscoleces) has reached (1 : 256) by using IHAT and (1 : 512)when use ELISA, The highest titter of third antigen (Germinal and Laminated layer)has reached (1 : 128) by using IHAT and (1 : 256) when we used ELISA method.
Conclusion: The highest antibodies titer could be found when use the Protoscoleces antigen more than Cyst fluid and Germinal and Laminated layer antigen.
American Psychological Association (APA)
Idan, Ikhlas Mushrif& Ardalan, Nakshin Mirza Mahmud. 2009. Estimation of humural immune response on the rabbits that immunizing with hydatid cyst antigens by using IHAT and ElISA. Journal of the Faculty of Medicine Baghdad،Vol. 51, no. 3, pp.332-335.
https://search.emarefa.net/detail/BIM-380500
Modern Language Association (MLA)
Idan, Ikhlas Mushrif& Ardalan, Nakshin Mirza Mahmud. Estimation of humural immune response on the rabbits that immunizing with hydatid cyst antigens by using IHAT and ElISA. Journal of the Faculty of Medicine Baghdad Vol. 51, no. 3 (2009), pp.332-335.
https://search.emarefa.net/detail/BIM-380500
American Medical Association (AMA)
Idan, Ikhlas Mushrif& Ardalan, Nakshin Mirza Mahmud. Estimation of humural immune response on the rabbits that immunizing with hydatid cyst antigens by using IHAT and ElISA. Journal of the Faculty of Medicine Baghdad. 2009. Vol. 51, no. 3, pp.332-335.
https://search.emarefa.net/detail/BIM-380500
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 335
Record ID
BIM-380500