Cytokine loealization in the gingiva and periodontal ligament of induced-diabetic rats, treated with ozone

Abstract EN

Objective : The aim of the present work was to study the effect of alloxan induced diabetes on the gingiva and periodontal ligament of rats under systemic ozone therapy through histological evaluation using hematoxylin and eosin and Masson’s trichrome stains and through immunohistochemical localization of interleukin-1 beta using avidin-Biotin peroxidase system with IL-lp mouse monoclonal antibody.

Material and Methods : Forty adult male albino rats were divided into 4 equal groups (10 animals each) ; group I : served as controls, group II were treated with ozone-oxygen (0302) gas mixture, group III : received alloxan for the induction of diabetes mellitus, and group IV were treated with alloxan to induce diabetes mellitus as in group III then received (0302) gas mixture.

At the end of the experiment, the animals of the different groups were sacrificed; the upper and lower jaws were dissected free.

Gingival specimens were taken from the first molar region of the jaws, fixed, and processed to be embedded in paraffin and then sectioned.

Jaw specimens were fixed, decalcified and processed to be examined histologically and immunohistochemically.

Results : Histopathological changes in the surface epithelium and lamina propria of the gingiva and periodontal ligament of the diabetic rats as well as marked increase in the cytokine interleukin-lp were found.

Conclusion : Administration of ozone-oxygen gas mixture to the diabetic rats resulted in marked recovery and improvement of the structures of the gingiva and periodontal ligament with a trial of the cytokine interleukin-1 (3 to return to normal.