الكشف عن المواد الشبيهة بالجبرلينات و الاكسينات و هرمون الأثلين في راشح نمو ثلاث عزلات محلية من الفطر Trichoderma harzianum

Abstract EN

Xhis study was conducted to detect the efficiency of three local isolates of Trichoderma harzianum (X9, X26 and X28) in producing some plant growth enhancement hormones like gibberellins, auxins and ethylene.

Xhe results showed the ability of the three tested isolates X9, X26 and X28 in producing gibberellins and auxins when cultivated on potato sucrose broth medium for 14 days on 25 ± 2 °C, but their production ability was varied significantly in producing the two studied hormones.

Xhe results of chemical analysis of culture filtrates using (HPLC) High-Performance Liquid Chromatography technique showed the superior of isolate X9 in their ability to produce gibberellins compared to X26 and X28 which recorded 43.39 mg / L compared to 18.24 ana 0.59 mg / L for isolates X26 and X28 respectively while isolate X26 recorded high efficiency in production auxins 22.3 mg / L compared to the productivity of isolate X9 and X28 which recorded 0.00 and 0.06 mg/L respectively.Gas chromatography technique showed the occurrence of ethylene in different quantities in the cultures of the three tested isolates of T.

harzianum cultivated on Czapek’s broth medium for four days on 25 ± 2 °C.

Xhis results confirmed the hypothesis of phytohomones production which enhance plant growth by some isolates of Trichoderma spp to be used as an important tool for plant growth enhancement.