Comprehensive study of in vitro fertilization of local goat

Abstract EN

-Goat ovaries brought from local abattoir within two hour of slaughter at 30° C , oocytes were asprated and then washed in Phosphate Buffer Solution(PBS )for cumulus - oocyte complexes (COCs) removed and these oocytes were divided in to two groups (with and with out cumulus) .

Granulated cytoplasm were selected and divided randomly t035 - 40 oocytes in 4-well dishes and incubated for 27 hours at 38.5° C with 5% CO2 atmosphere in air with 95% humidity .

In vitro fertilization were performed after maturation oocytes by capacitated fresh sperms taken by artificial vagina from proven fertile bucks , by addition of a microdrops (1  106 sperm) and culturing with matured oocytes for 24 hours at the same environement mentioned above.

The large gravian folliculs (g.fs.) had more identified oocytes than the small ones.