Characterization of Toxocara canis excretory secretory antigen and its application for diagnosis of clinically suspected cases of ocular toxocariasis by western blot analysis
Other Title(s)
تحليل المحفز المناعي للتوكسوكارا كانيز و استعماله في تشخيص حالات اليرقة المهاجرة بالعين (المتوقعة إكلينيكياً) بواسطة الوسترن بلوت
Joint Authors
Ali, Umar Muhammad
Shahin, Maha Sayyid Ibrahim
Source
Issue
Vol. 30, Issue 3 (30 Sep. 2006), pp.237-246, 10 p.
Publisher
Assiut University Faculty of Medicine
Publication Date
2006-09-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Topics
Abstract EN
Toxocariasis is a frequent helminthiasis that can cause visceral and ocular damage in humans.
The present work represents three cases of suspected ocular toxocariasis(OT) .
complaining of visual impairment.
Ocular toxocariasis was suspected based on clinical manifestations.
Diagnosis was confirmed by Western blot analysis.
The identification of specific antigen of Toxocara canis is important in order to develop better diagnostic techniques .
Toxocara excretory secretory antigen (TES) was prepared and it was characterized by SDS-PAGE.
A characteristic pattern of nine bands was achieved .
These bands were at (17, 26, 32 , 35,45, 55, 70, 90,and 120) KDci.
Components ofT.
canis ES antigen (TES) were identified by sera of clinically suspected cases of ocular toxocariasis (OT) by using (WB) analysis.
The identified proteins were grouped into one low molecular weight fraction (26-35 KDa) and one high molecular weight fraction (55-90 KDa).
These antigenic bands could be considered as a guide for the diagnosis of ocular toxocariasis.
Systemic albendazole way given conjunction with steroids.
Treatment resulted in an improved visual acuity in all patients.
American Psychological Association (APA)
Shahin, Maha Sayyid Ibrahim& Ali, Umar Muhammad. 2006. Characterization of Toxocara canis excretory secretory antigen and its application for diagnosis of clinically suspected cases of ocular toxocariasis by western blot analysis. Assiut Medical Journal،Vol. 30, no. 3, pp.237-246.
https://search.emarefa.net/detail/BIM-38520
Modern Language Association (MLA)
Shahin, Maha Sayyid Ibrahim& Ali, Umar Muhammad. Characterization of Toxocara canis excretory secretory antigen and its application for diagnosis of clinically suspected cases of ocular toxocariasis by western blot analysis. Assiut Medical Journal Vol. 30, no. 3 (Sep. 2006), pp.237-246.
https://search.emarefa.net/detail/BIM-38520
American Medical Association (AMA)
Shahin, Maha Sayyid Ibrahim& Ali, Umar Muhammad. Characterization of Toxocara canis excretory secretory antigen and its application for diagnosis of clinically suspected cases of ocular toxocariasis by western blot analysis. Assiut Medical Journal. 2006. Vol. 30, no. 3, pp.237-246.
https://search.emarefa.net/detail/BIM-38520
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 244-246
Record ID
BIM-38520