Extraction and immobilization of hemoysin a produced by uropathogenic Escherichia coli

Abstract EN

Out of the 300 urine samples, only 47(15.7%) isolates were identified as Escherichia coli.

The hemolysin produced by E.coli was studied, and it was found that 19(40.4%) out of the 47 isolates of E.coli were able to produce hemolysin extracellularly on human and rabbit blood agar.

Hemolysin extraction was done and five hemolysin filtrates were subjected to purification, which was treated with ammonium sulfate to obtain 20-40% saturation in phosphate buffer (0.05 M, pH: 6.8).

It was found that all of these filtrates contained hemolytic activity at a level higher than that before purification.

Immobilization of both hemolysin producing bacteria and free purified hemolysin was also done.

It was seen that immobilization by sodium alginate had no effect on hemolysin productivity and activity and the immobilized hemolysin retained its activity after re-using twice or more on blood agar.

The effect of purified hemolysin on WBCs in human blood samples and urine blood samples was also studied.

It was seen that there were significant effects of hemolysin samples on WBCs count which caused reduction in the number of these cells in both blood samples and in urine samples.