Methylenetetrahydrofolate reductase C677T and a1298C polymorphism in Iranian women with idiopathic recurrent pregnancy losses

Abstract EN

Background : Recurrent pregnancy loss (RPL) is a serious problem for pregnancy.

There is evidence that vascular complications play a principal role in RPL.

Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in folate metabolism.

Polymorphisms (C677T, A1298C) of MTHFR gene are associated with decreased MTHFR activity.

Objectives : The aim of this study was to determine the association between MTHFR polymorphisms (C677T and A1298C) and recurrent pregnancy loss (RPL) in Iranian women.

Materials and Methods : In this case-control study, blood samples were obtained from patients who had three or more consecutive pregnancy losses before the 22nd week of pregnancy (n = 204).

The control group consisted of 116 age-matched women with at least one alive child and without any history of pregnancy loss or other gestational complications (n = 116).

Following DNA extraction, samples were tested for MTHFR C677T and A1298C polymorphisms using the reverse hybridization method.

Results : The prevalence of 677TT mutation was 8.8% (18 / 204) in the patient group and 8.6 % (10 / 116) in the control group (P = 0.434).

The prevalence of 1298CC mutation was 12.3 % (25 / 204) in the patient group and 8 % (9 / 116) in the control group (P = 0.155).

Investigation of the distributions of various genotypes of MTHFR C677T and A1298C did not indicate a significant difference between patients with RPL and healthy control subjects.

Conclusions : The results suggest that MTHFR mutations might not be associated with RPL in the examined population.