Biological activity of some moulting hormone agonists in mealworms : ecdysteroid and protein analysis in ovaries
Joint Authors
Sultani Mazuni, N.
Bu Qshaybah, A.
Smagghe, G.
Source
Issue
Vol. 2004, Issue 13 (31 Jul. 2004), pp.7-10, 4 p.
Publisher
Annaba Badji Mokhtar University
Publication Date
2004-07-31
Country of Publication
Algeria
No. of Pages
4
Main Subjects
Abstract EN
In a topical (10 µg/f'cmale) bioassay, three moulting hormone agonists (RH-0345, RH-5849, RI1-5992) were tested on newly emerged adult females of the mealworm, Tenebrio molitor (Coleoptera: Tenebrionidae), under in vivo conditions.
In a first series of experiments the compounds were assayed on ovarian ecdysteroid production measured at day 2 following treatment, corresponding to the beginning of vitellogenesis.
Data from enzyme immunoassay measurements revealed that all tested compounds resulted in a significant increase in ovarian ecdysteroid amounts.
Moreover, RH-5992 was found more active than RH-0345 and RH-5849.
In a second series of experiments, the activity of these compounds was investigated on ovarian proteins.
The ecdysteroid agonist RI 1-5849 caused a significant reduction of protein amounts in the ovaries.
The electrophoretic separation of ovarian extracts from treated series showed that one or two protein bands were missing compared to controls.
These observations may suggested an interference of ecdysteroid agonists with the vitellogenesis process.
Abstract FRE
Trois mimétiques de l’hormone de mue (RH-0345, RH-5849, RH-5992) ont etc testés par application topique (10 |j.g/feme!!e) sur les adultes femelles de Tenehrio molitor (Coleoptera: Tcnebrionidae), en conditions in vivo.
Dans une première série d'expériences, les composés ont été évalués sur la production d’ecdystéroïdes ovariens mesurée à l’âge deux jours après traitement correspondant au début du processus de la vitellogénèse.
I.es résultats des dosages enzymo-immunologiques des hormones révèlent que tous les produits testés provoquent une augmentation significative des taux ovariens d’ecdystéroïdes.
De plus, RH-5992 et RH-0345 sont plus actifs que RH-5849.
Dans une seconde expérimentation, l’effet de ces composés a été examine sur les protéines ovariennes.
Seul le RH-5849 réduit significativement le taux de protéines dans les ovaires.
La séparation éiectrophorètique des extraits ovariens montre l’absence d’une ou de deux fractions proteiques chez les séries traitées comparativement aux témoins.
Ces observations suggèrent une interférence des agonistes des eedystéroïdes avec le processus de vitellogénèse.
American Psychological Association (APA)
Sultani Mazuni, N.& Bu Qshaybah, A.& Smagghe, G.. 2004. Biological activity of some moulting hormone agonists in mealworms : ecdysteroid and protein analysis in ovaries. Synthèse،Vol. 2004, no. 13, pp.7-10.
https://search.emarefa.net/detail/BIM-388452
Modern Language Association (MLA)
Sultani Mazuni, N.…[et al.]. Biological activity of some moulting hormone agonists in mealworms : ecdysteroid and protein analysis in ovaries. Synthèse No. 13 (Jul. 2004), pp.7-10.
https://search.emarefa.net/detail/BIM-388452
American Medical Association (AMA)
Sultani Mazuni, N.& Bu Qshaybah, A.& Smagghe, G.. Biological activity of some moulting hormone agonists in mealworms : ecdysteroid and protein analysis in ovaries. Synthèse. 2004. Vol. 2004, no. 13, pp.7-10.
https://search.emarefa.net/detail/BIM-388452
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 10
Record ID
BIM-388452
