Effect of reduced dietary zinc and experimental diabetes on GOT, GPT and alkaline phosphatase activities in rats

Abstract EN

To investigate the effect of low dietary zinc intake and experimental diabetes (lDDMJ on transaminases and alkaline phosphatase activities in rats, 8 weeks old male wealing normal albino (Wtstar) rats were fed UTI semi-synthetic diet containing either adequate (54 mglkg) or deficient (/ mglkg) quantities of zinc for one week.

Ten rats from each group (n=20) were then intraperitoneally injected with alloxan to induce diabetes.

The rats were sacrificed after further three weeks.

Body weight gain and food intake were recorded regularly.

On day 28 after an overnight fast, animals were killed and blood glucose, serum zinc, serum glutamic oxalic transaminase (GOT), serum glutamic pyruvic transaminase (GPT) and serum alkaline phosphatase were determined.

Body weight gain of diabetic animals at the end of four weeks of dietary manipulation was significantly lower than those of/he non-diabetic animals.

Compared with non-diabetic rats both diabetic groups had higher food intake and lower serum zinc.

Dietary zinc intake did not significantly alter the body weight gain.

total food intake and serum zinc of diabetic or non-diabetic rats.

In alloxan diabetes serum (GOT), (GPT) were Significantly .increased compared to normal rats, while the level of serum alkaline phosphatase was decreased.

The consumption of low-Zn diet led to increasing of GOT.

GPT.

However, serum alkaline phosphatase was decreased.