An association between GSTM1 and GSTT1 gene polymorphism with some physiological parameters among haemodialysis patients in al-Qadisiya Province Iraq

Abstract EN

Several studies have shown that high oxidative stress levels are associated with haemodialysis (HD) .

Glutathione S- transferase ( GSTM1 and GSTT11) are members of the GST family of proteins , which protects cellular DNA against oxidative damage .

This study tested the association of GSTM1 and GSTT1 gene polymorphism with some physiological parameters among haemodialysis patients.GSTT1 and GSTM1 genotypes and the estimations of some biomarkers were determined in 60 HD patients and 60 age- and gender – matched controls .There was a significant difference between males and females ( P ˂ 0.05, χP2 Ptest) patient group.

The biomarkers results of this study (the mean haemoglobin (Hb) concentration, packed cell volume (PCV) and plasma glutathione (GSH) level) were significantly lower (P < 0.05, χP2 Ptest) in HD patients in comparison with healthy subjects, while levels of Urea, Creatinine, malondialdehyde (MDA) and GST were significantly higher (P < 0.05) in HD patients compared with control.

We observed that GSTM1 (-) null genotype was present in 50% of the HD patients, While GSTT1 (-) null genotype was present in 33.33% of the HD patients.

There was a significant association of null alleles of the GSTM1 (-) (P= 0.031, OR = 3.1, 95% CI = 25.0-27.14) and GSTT1 (-) ( P = 0.015 , OR= 1.75, 95 % CI = 34.82-46.82) with HD patients.

The combinations of GSTM1 (-) and GSTT1 (-) null genotype were (33.33 %) in the HD patients, the difference was significant (P = 0.032 OR = 2.58 , 95 % CI = 32.11- 41.31 ) .

It was concluded that there is an association between GSTT1 and GSTM1 gene polymorphisms and oxidative stress leading to functional deficiency in in haemodialysis patients .