Association of HLADQA1 and HLADRB1 alleles with asthma patient in Basra City

Abstract EN

The current study were included one hundred and twenty blood sample with draw form patients with allergic asthma , they were selected randomly from the Allergy and Asthma Center in Basra cityand there were eighty sample with draw from healthy individual as control .

All samples from patients and controls were tested by direct ELISA for total IgE and Polymerase chain reaction was performed to detect a genetic relationship between allergic asthma and human leukocyte antigen classII allele(HLA- DQA1 and HLA- DRB1 alleles).Result of this study showed that the total IgE > 100 IU / ml had higher rate in asthma patients at (70.8%).with significant difference( p < 0.05) between total IgE < 100 IU / ml and controls.

The study also included detection of some human leukocyte antigens(HLAclassII) allelesHLA DRB1*0101 / 2 /4, HLA DQA1*0401, HLA DQA1*0101 / 4 by the use of PCR analysis to determine their impact on the asthma allergy.

The results of this analysis revealed the HLA DQA1*0401 had higher rate at(89.2 %) inasthma patients ,also this allele appeared in 100% of asthma patientswho had total IgE 100 IU / ml .

All studied HLA class II alleles were significantly (P<0.05) distributed in the study groups .