Development of a cell culture attenuated vaccine against lumpy skin disease part (1)‎ : pathogenicity and immunogenicity of high culture-passaged viruses

Abstract EN

After 54 passages in bovine kidney cell (BKC) culture and clonal selection, a Sudancese isolate of "Neethling" type virus, designated "Massalamia", though found to be capable of protecting zebu cattle against virulent challenge of lumpy skin disease virus (LSDV), could produce adverse post-vaccinal clinical reaction and it grew poorly in BKC cutlure.

However, after 66th passages and clbnai selection it grew vigorously in BKC culture and caused no such adverse effects, yet it was still capable of protecting zebu cattle against challenge of the virus.

All animals vaccinated with either passage levels were hypersensitive to an inactivated LSD viral antigen and to the intradermal LSDV challenge, while only those with post-vaccinal localized reaction developed in addition a significant neutralizing antibody response.

The antibody response to the 54th passage level was higher than which followed vaccination with the 66th passage level irrespective of the dose of the virus inoculated