In vitro propagation of the medicinal plant Ziziphora tenuior L. and evaluation of its antioxidant activity
Joint Authors
Sulayman, Muhammad
Dakah, Abd al-Karim
Zayd, Salim
Abbas, Sami
Wink, Michael
Source
Saudi Journal of Biological Sciences
Issue
Vol. 21, Issue 4 (30 Sep. 2014), pp.317-323, 7 p.
Publisher
Publication Date
2014-09-30
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
Ziziphora tenuior L.
(Lamiaceae) is an aromatic herb used for its medicinal values against fungi, bacteria.
Micropropagation can be used for large-scale multiplication of essential oil producing plants thus avoiding an overexploitation of natural resources.
This work aims to develop a reliable protocol for the in vitro propagation of Z.
tenuior, and to compare the antioxidant activity between in vitro propagated and wild plants.
The explants were sterilized and cultured on MS medium containing different concentrations of growth regulators naphthalene acetic acid (NAA) or indole-3-butyric acid (IBA) with 0.5 mg/L of kinetin (Kin) callus formation was 70.2% after 45 days of incubation in dark on medium supplemented with 1.5 mg/L of NAA.
After one month of callus culture on medium supplemented with 2 mg/L BA the shoot number was 5.12 and for the multiplication stage.
The shoot number was 4.21 and length was 6.17 cm on medium supplemented with 1 mg/L Kin +0.1 mg/L NAA.
DPPH reagent was used to test the antioxidant activity.
The aqueous and methanol extracts of in vitro plants which were treated with 1.5 and 1 mg/L of kin plus 0.1 mg/L of NAA showed a strong DPPH scavenging activity where IC50 was 0.307 and 0.369 mg/ml, respectively, while the IC50 of aqueous and methanol extracts of wild plants was 0.516 and 9.229 mg/ml, respectively.
Our results suggested that plant growth regulators and in vitro culture conditions increased the antioxidant activity.
American Psychological Association (APA)
Dakah, Abd al-Karim& Zayd, Salim& Sulayman, Muhammad& Abbas, Sami& Wink, Michael. 2014. In vitro propagation of the medicinal plant Ziziphora tenuior L. and evaluation of its antioxidant activity. Saudi Journal of Biological Sciences،Vol. 21, no. 4, pp.317-323.
https://search.emarefa.net/detail/BIM-393859
Modern Language Association (MLA)
Wink, Michael…[et al.]. In vitro propagation of the medicinal plant Ziziphora tenuior L. and evaluation of its antioxidant activity. Saudi Journal of Biological Sciences Vol. 21, no. 4 (Sep. 2014), pp.317-323.
https://search.emarefa.net/detail/BIM-393859
American Medical Association (AMA)
Dakah, Abd al-Karim& Zayd, Salim& Sulayman, Muhammad& Abbas, Sami& Wink, Michael. In vitro propagation of the medicinal plant Ziziphora tenuior L. and evaluation of its antioxidant activity. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 4, pp.317-323.
https://search.emarefa.net/detail/BIM-393859
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 321-323
Record ID
BIM-393859