Changes in the pattern of protein in the haemolymph and fat body of the pink bollworm Pectinophora gossypieua saunders during the active and diapause phases of the larva

Abstract EN

The concentration of total protein in the haemolymph and fat body of the fourth instar larva of Pectinophora gossypiella Saunders increased in diapause and in early ages of terminationofdiapause relative to that in the active phase.

In late ages of termination of diapause, a drop took place.

Thehaemolymph and fat body of the active, diapause and7 agesduring termination of diapause of the larva were electrophoretically separated into 15 and 12 protein bands for both tissues, respectively.

The different phases of the larva showed 2-7 protein bands whose concentration varied greatly during induction and termination ofdiapause.

Some of these bands in both tissues examined were simple proteins, others were glycoproteins, lipoproteins or lipoglycoproteins.

Some of these bands in both tissues were identical, while others were non-identical or specificior each tissue.

During diapause, 3 characteristic protein bands appeared in each of the haemolymph and fat body, of which only one band was identical in both tissues.

During termination of diapause some protein bands appeared in the haemolymph before their appearance in the fat body., or were neyer detected in the fatbody, which suggested that some tissues, other than the fat body, might be involved in synthesizing haemolymph protein.

The haemolymph and fat body during late ages of diapause termination regained many of the characteristic protein pattern of the active phase.