Tobacco leaf proteins and effect of infection with tobacco vein mottling virus and tobacco mosaic virus on their concentrations

Abstract EN

-Total prote ins , alb umi ns .

globulins .

glut olin s.

prolam in s and amino acid s of resid ual prot e ins were determined in he alth y and tobacco ve in mottling virus (TYMY) or to bacco mo saic virus (T MY) in fec ted tobacco i Nicotiana tabacum L.

) lea ves.

T he to ta l p rot ein s we re abo ut 40'}'o of the dr y we ight of healthy le aves .

Th e so luble p rot e ins were 28,6% of th e tot al prote ins a nd most of which co nsisted of albumins an d globulins.

T obacco le aves infected with TMY co nt ained significa nt ly mor e to tal pro te ins but sol ub le protei n fraction s were not significant ly alte red .

The TYMY infect ed leaves co ntained less alb umins a nd mo re globulins th an he althy le aves.

Th e TMY infect ed leaves were not significa ntly d iffe re nt fro m he a lthy leaves in glo bulins and albumins conce ntra tio n.

Nine amino acids were dete cte d in th e re sidual protei ns .

The co ncentra tio n o f amino acids were highe r in TYMY infe cted leaves th an th at ob ta ined in he althy lea ves.

Of th e 9 am ino acids de tected o nly 7 wer e highe r in TM Y infe ction s.

How e ver .

ove ra ll in cre ase in amino acid s conce ntra tio n was highe r in TMY infec tion s than that with TYMY.