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In Vitro activity of novel metronidazole derivatives on larval stages of Echinococcus granulosus
Joint Authors
Saadah, Haytham A.
Mubarak, Muhammad S.
Nasr, Wafa T.
Abd al-Hafiz, Sami K.
Source
Jordan Journal of Biological Sciences
Issue
Vol. 7, Issue 3 (30 Sep. 2014), pp.187-194, 8 p.
Publisher
The Hashemite University Deanship of Academic Research and Graduate
Publication Date
2014-09-30
Country of Publication
Jordan
No. of Pages
8
Main Subjects
Topics
Abstract EN
The effects of metronidazole (MTZ) and novel synthesized MTZ derivatives on in vitro cultured Echinococcus granulosus protoscoleces (PSCs), 30 day old segmentation stage and hydatid cysts (HC) developing secondarily in BALB/c mice were compared to those caused upon treatment with comparable doses of albandazole (ABZ) and mebendazole (MBZ) drugs.
The highest protoscolicidal action resulted from the use of a non-schiff based MTZ derivative (MTZ-w: 4-[2-(2-methyl-5-nitro-lH-imidazol-l-yl) ethyoxy] benzeyldehyde).
Incubation of PSCs with MTZ-w concentrations of 25, 12.5 and 6.25 ^g/ml resulted in significantly higher mortality rates than those caused by ABZ or MBZ at all periods post incubation.
Total mortality of PSCs always occurred one day earlier using MTZ-w.
Moreover, incubation of PSCs with MTZ-w at 6.25 ^g/ml concentration resulted in greater mortality of PSCs than that caused by ABZ at 25ug/ml concentration.
Three other MTZ derivatives showed similar in vitro effects on PSCs to those caused by ABZ or MTZ.
Light microscopy revealed that changes in PSCs exposed to MTZ derivatives and ABZ reflected their relative actions in targeting scolex hooks, suckers and tegument.
MTZ-w and ABZ caused rupture of hooks, deformation in suckers and disintegration in tegument of both PSCs and in vitro cultured segmentation stage.
Less detrimental changes occurred upon the exposure to other MTZ derivatives.
Exposure of HC to MTZ-w and ABZ caused regression in their size, damage in germinal membrane, fragmentation of underlining tissue, and scaling of laminated membrane.
MTZ-w warrants further assessment as a potential chemotherapeutic drug against cystic echinococcosis in both animals and humans.
American Psychological Association (APA)
Nasr, Wafa T.& Saadah, Haytham A.& Mubarak, Muhammad S.& Abd al-Hafiz, Sami K.. 2014. In Vitro activity of novel metronidazole derivatives on larval stages of Echinococcus granulosus. Jordan Journal of Biological Sciences،Vol. 7, no. 3, pp.187-194.
https://search.emarefa.net/detail/BIM-399286
Modern Language Association (MLA)
Nasr, Wafa T.…[et al.]. In Vitro activity of novel metronidazole derivatives on larval stages of Echinococcus granulosus. Jordan Journal of Biological Sciences Vol. 7, no. 3 (Sep. 2014), pp.187-194.
https://search.emarefa.net/detail/BIM-399286
American Medical Association (AMA)
Nasr, Wafa T.& Saadah, Haytham A.& Mubarak, Muhammad S.& Abd al-Hafiz, Sami K.. In Vitro activity of novel metronidazole derivatives on larval stages of Echinococcus granulosus. Jordan Journal of Biological Sciences. 2014. Vol. 7, no. 3, pp.187-194.
https://search.emarefa.net/detail/BIM-399286
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 193-194
Record ID
BIM-399286