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Measurements of homogentisic acid levels in alkaptonuria patients using an optimized and validated gas chromatography method mass spectrometry
Joint Authors
al-Sbou, Muhammad S.
al-Sarayirah, Samih A.
al-Tarawinah, Ibrahim N.
al-Batayinah, Iman M.
al-Shuneigat, Jihad M.
al-Sarayirah, Yusuf M.
Source
Jordan Journal of Biological Sciences
Issue
Vol. 7, Issue 3 (30 Sep. 2014), pp.195-198, 4 p.
Publisher
The Hashemite University Deanship of Academic Research and Graduate
Publication Date
2014-09-30
Country of Publication
Jordan
No. of Pages
4
Main Subjects
Topics
Abstract EN
Alkaptonuria is a very rare genetic disorder, characterized by a lack of homogentisate dioxygenase and causes accumulation of homogentisic acid.
Clinical manifestations include dark urine, dark-black pigmentation of connective tissues (ochronosis), and arthritis of large joints and spine.
The disease is usually diagnosed in adulthood by assessment of signs and symptoms of ochronosis.
Confirmation of suspected diagnosis can be achieved by quantitative measurements of homogentisic acid levels.
In this study, homogentisic acid was analyzed in 17 alkaptonuria patients.
After liquid-liquid extraction, the analyte was determined by Gas Chromatography/ Mass Spectrometry (GC-MS) method.
For quantitation purposes, external calibration was applied first, regression coefficient of > 0.995 indicated the linearity in the concentration range of 1-100 ng/^l.
The instrumental detection limit (IDL) and lower limit of quantitation were 3.82 and 12.7 ^g/L, respectively.
Recovery rate was > 89%.
Precision given as relative standard deviation (RSD) ranged from 3 - 10 %.
The results showed that the concentration of homogentisic acid ranged from 0.46 to 1.5 g/24 hours.
American Psychological Association (APA)
al-Sarayirah, Samih A.& al-Tarawinah, Ibrahim N.& al-Sbou, Muhammad S.& al-Batayinah, Iman M.& al-Shuneigat, Jihad M.& al-Sarayirah, Yusuf M.. 2014. Measurements of homogentisic acid levels in alkaptonuria patients using an optimized and validated gas chromatography method mass spectrometry. Jordan Journal of Biological Sciences،Vol. 7, no. 3, pp.195-198.
https://search.emarefa.net/detail/BIM-399293
Modern Language Association (MLA)
al-Shuneigat, Jihad M.…[et al.]. Measurements of homogentisic acid levels in alkaptonuria patients using an optimized and validated gas chromatography method mass spectrometry. Jordan Journal of Biological Sciences Vol. 7, no. 3 (Sep. 2014), pp.195-198.
https://search.emarefa.net/detail/BIM-399293
American Medical Association (AMA)
al-Sarayirah, Samih A.& al-Tarawinah, Ibrahim N.& al-Sbou, Muhammad S.& al-Batayinah, Iman M.& al-Shuneigat, Jihad M.& al-Sarayirah, Yusuf M.. Measurements of homogentisic acid levels in alkaptonuria patients using an optimized and validated gas chromatography method mass spectrometry. Jordan Journal of Biological Sciences. 2014. Vol. 7, no. 3, pp.195-198.
https://search.emarefa.net/detail/BIM-399293
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 198
Record ID
BIM-399293