Changes in protein contents of resistant and susceptible barley coleoptiles in response to inoculation with Ustilago hordei race 6

Abstract EN

Sccds of barl cycul t ivars Pan nic rtr"} .

l.BO) a nd Odes" , (C.

I .

lJ3.t) ( resistant and susceptible 10 cove red SIll UI di se ase .

respect ivel y} were ge rminate d .

an d the ir co lco ptilcs were inoc ulat ed with Ustilago hordei race (1.

T he coleoptiles were ha rvested 1, 2 antl .l days inter va ls afte r inocul ation and the to ta l soluble prot e ins a lbumins and glob ulinc s we re es timate d .

T he co lo rmetric assay showed t hat mos t (If inocul at ed co lco pt iles co nta ine d less so luble p rot ei ns th an the non-ino cula ted co lco pt ilcs , rega rd less o f culn var s e xam ined.

Howeve r .

th e alb umin co nte nts in bo th th e cult iva rs we re highe r significa ntly in the inocu lated co lco ptilcs than in the non -inocul at ed .

T he globulin co ntent incr ease d in th c ino cula te d tissues o f bo th culti vars , Th e incr e ase in globulin co nte nts were high er in the resistan t IT Pannie r tha n in the susce pt ible (' I' Odessa at t hre e days after inocu lation s tage .

The glo bulin fraction s o f Pannie r and Odessa co lcopti lcs sepa rated in to 12 and 0 clc tro pho rctic bands.

re spect ive ly.

Tb e den sity o f globulin ba nds in barley co lcopt ile»were generally increased in response to inoculation with U.

hordci .