High performance liquid chromatographic method for the determination of diclofenac sodium in pharmaceutical preparations and in environmental samples

Abstract EN

Rverse phase high performance liquid chromatography (HPLC) method has been developed for the determination of diclofenac sodium in pharmaceutical preparations and environmental water samples.

Chromatography was carried out on supelco C18 column (25cm × 4.6m.m), 5μm.

Using methanol as mobile phase.

The flow rate was 1.0 ml / min with Uv-detection at 283 nm.

Separation was completed within 2.9 min.

Calibration curve was linear coefficient correlation 0.998 over a concentration range of (5-80μg / ml).

The relative standard deviation (RSD) was found < 1 %.

The proposed method was successfully applied to the determination of diclofenac sodium in pharmaceutical dosage forms and in environmental water samples.