Effect of in vitro activation byglycyrrhiza glabra extract on cryostorage

Abstract EN

The aim of the present study is to investigate the effect of Glycyrrhiza glabra extract on certain sperm function parameters using in vitro activation technique for cryostorage of buck semen following several days of ejaculation (1, 2 and 3 days).

Eight mature indigenous bucks aged (2-3 years) were used.

Semen was collected once a week from each male using artificial vagina.

The semen diluted by using modified Tris solution (MTS) (Tris 3.03gm + fructose 1.25gm + citric acid 1.67gm + egg yolk) then stored in refrigerator (4-5) °C for 24, 48, & 72 hours and study the effect of adding Gg in modified culture media using simple layer technique.

The results of certain sperm function parameters of cryostorage sperm were recorded.

The results showed that Adding 20 % Gg in modified culture media and in vitro activation for 60 minutes had a significant improvement of sperm motility and grade activity of progressive forward movement following several days of ejaculation.

There was a significant decrease in values of sperm concentration after activation compared with that before activation technique.

There was no significant (p > 0.05) differences in the percentage of morphologically normal sperm (MNS) between before and after activation technique.

These results were attributed to the constituents of Gg such as vitamins, minerals, estrogenic and anti estrogenic substance, trace element, and polysaccharide which have effect on certain sperm parameters.

The results of this study indicate that using Gg for in vitro activation of preserved buck semen improve some sperm function parameters, in which it can be utilized for artificial insemination.