Spectrophotometric (flow injection and batch)‎ methods for the determination of some catecholamine drugs in pharmaceutical preparations

Abstract EN

A batch and flow injection (FIA) Spectrophotometric methods have been investigated for the determination of some catecholamine drugs in aqueous solution and in pharmaceutical preparations.

The methods are based on the reaction of catecholamine drugs ( methyl dopa (I), adrenaline (П) and dopamine (Ш)) with N, N-dimethyl aniline in the presence of potassium metaperiodate in neutral media to form an orange water soluble stable dye that has a maximum absorption at λmax 478,493 and 482nm for (I), (П) and (Ш) respectively .

linearity was in the range of 1-40,0.5-30 and 0.5-20 μg.ml-1 with a limit of detection ( signal / noise = 3) of 0.39, 0.34 and 0.36 μg.ml-1 for (I), (П) and (Ш) respectively by batch method and 5-60, 5-100 and 5-100 μg.ml-1 with a limit of detection of 0.90, 0.88 and 1.71 μg.ml-1 for (I), (П) and (Ш) respectively by FIA method .The effect of chemical and physical parameters have been carefully considered and the proposed procedures were successfully applied to the determination of some catecholamine drugs in pharmaceutical formulation.