Detection of Epstein Barr virus lmp-1 gene in non Hodgkin lymphoma Iraqi patients

Abstract EN

Non-Hodgkin's lymphomas (NHLs) are hematologic malignancy with the highest prevalence worldwide.

They are broadly classified as B-cell or T-cell lymphoma depending on which type of lymphocyte becomes cancerous, B-cell lymphoma is more common than T-cell lymphoma.

The EBV infects certain epithelial cells and linked to the development of multiple cancers, including NHL, HL, and nasopharyngeal carcinoma.

This study designates to determine the frequency EBV lmp-1 inNHL Iraqipatients, and todetermine the correlation between EBV lmp-1 and subtypes of NHL.

A 42 FFPE blocks were examined included 32 blocks from NHL patients, and 10 blocks from reactive follicular hyperplasia.

Genomic DNA was extracted from these blocks using a specific kit and amplified by polymerase chain reaction (PCR) by using oligonucleotide primers specific for EBV lmp-1 gene.

Our result shows EBV lmp-1 was detected in 43.8 % cases of NHL, and in 10.0 % cases of reactive follicular hyperplasia with no significant (P > 0.0.5) variation between these groups.

In addition to that, incidence ofEBV lmp-1 was more frequently detected in high grade.

From these results we conclude that EBV lmp-1 plays an important role in NHL development especially in high grade histopathological type.