Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (chlorophyta) using ISSR and rbcL markers
Joint Authors
Wungsawad, Pheravut
Peerapornpisal, Yuwadee
Source
Saudi Journal of Biological Sciences
Issue
Vol. 21, Issue 5 (30 Nov. 2014), pp.505-510, 6 p.
Publisher
Publication Date
2014-11-30
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
Spirogyra is found in a wide range of habitats, including small stagnant water bodies, rivers, and streams.
Spirogyra ellipsospora is common in northern Thailand.
Species identification of the Spirogyra species based only on morphological characteristics can be difficult.
A reliable and accurate method is required to evaluate genetic variations.
This study aims to apply molecular approaches for the identification of S.
ellipsospora using microsatellites and rbcL markers.
Based on DNA sequencing, the rbcL gene was sequenced and the data was analyzed using the BLAST (Basic Local Alignment Search Tool) program in the NCBI (National Center for Biotechnology Information) database.
The sequence of S.
ellipsospora from this study revealed definitive identity matches in the range of 99% for the consensus sequences of S.
ellipsospora.
The 10 primers of ISSR could be amplified by 92 amplification fragments.
The DNA fragments and the rbcL sequence data grouped the Spirogyra specimens into two distinct clusters.
American Psychological Association (APA)
Wungsawad, Pheravut& Peerapornpisal, Yuwadee. 2014. Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (chlorophyta) using ISSR and rbcL markers. Saudi Journal of Biological Sciences،Vol. 21, no. 5, pp.505-510.
https://search.emarefa.net/detail/BIM-409903
Modern Language Association (MLA)
Wungsawad, Pheravut& Peerapornpisal, Yuwadee. Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (chlorophyta) using ISSR and rbcL markers. Saudi Journal of Biological Sciences Vol. 21, no. 5 (Nov. 2014), pp.505-510.
https://search.emarefa.net/detail/BIM-409903
American Medical Association (AMA)
Wungsawad, Pheravut& Peerapornpisal, Yuwadee. Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (chlorophyta) using ISSR and rbcL markers. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 5, pp.505-510.
https://search.emarefa.net/detail/BIM-409903
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 510
Record ID
BIM-409903