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Identification and phylogeny of Arabian snakes : comparison of venom chromatographic profiles versus 16S rRNA gene sequences
Joint Authors
Khan, Hasib Ahmad
Mundhiri, Raja Muhammad Abbas
al-Asmari, Abd al-Rahman K.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 21, Issue 5 (30 Nov. 2014), pp.436-441, 6 p.
Publisher
Publication Date
2014-11-30
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
Identification of snake species is important for various reasons including the emergency treatment of snake bite victims.
We present a simple method for identification of six snake species using the gel filtration chromatographic profiles of their venoms.
The venoms of Echis coloratus, Echis pyramidum, Cerastes gasperettii, Bitis arietans, Naja arabica, and Walterinnesia aegyptia were milked, lyophilized, diluted and centrifuged to separate the mucus from the venom.
The clear supernatants were filtered and chromatographed on fast protein liquid chromatography (FPLC).
We obtained the 16S rRNA gene sequences of the above species and performed phylogenetic analysis using the neighbor-joining method.
The chromatograms of venoms from different snake species showed peculiar patterns based on the number and location of peaks.
The dendrograms generated from similarity matrix based on the presence/absence of particular chromatographic peaks clearly differentiated Elapids from Viperids.
Molecular cladistics using 16S rRNA gene sequences resulted in jumping clades while separating the members of these two families.
These findings suggest that chromatographic profiles of snake venoms may provide a simple and reproducible chemical fingerprinting method for quick identification of snake species.
However, the validation of this methodology requires further studies on large number of specimens from within and across species.
American Psychological Association (APA)
al-Asmari, Abd al-Rahman K.& Mundhiri, Raja Muhammad Abbas& Khan, Hasib Ahmad. 2014. Identification and phylogeny of Arabian snakes : comparison of venom chromatographic profiles versus 16S rRNA gene sequences. Saudi Journal of Biological Sciences،Vol. 21, no. 5, pp.436-441.
https://search.emarefa.net/detail/BIM-409966
Modern Language Association (MLA)
al-Asmari, Abd al-Rahman K.…[et al.]. Identification and phylogeny of Arabian snakes : comparison of venom chromatographic profiles versus 16S rRNA gene sequences. Saudi Journal of Biological Sciences Vol. 21, no. 5 (Nov. 2014), pp.436-441.
https://search.emarefa.net/detail/BIM-409966
American Medical Association (AMA)
al-Asmari, Abd al-Rahman K.& Mundhiri, Raja Muhammad Abbas& Khan, Hasib Ahmad. Identification and phylogeny of Arabian snakes : comparison of venom chromatographic profiles versus 16S rRNA gene sequences. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 5, pp.436-441.
https://search.emarefa.net/detail/BIM-409966
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 441
Record ID
BIM-409966